Journal: Journal of Nuclear Medicine

Article Title: Clinical Translation of a 68 Ga-Labeled Integrin α v β 6 –Targeting Cyclic Radiotracer for PET Imaging of Pancreatic Cancer

doi: 10.2967/jnumed.119.237347

Figure Lengend Snippet: (A) Chemical structure of DOTA-cycratide. (B) Inhibition of 64Cu-cycratide binding to integrin αvβ6 on BxPC-3 cells by cycratide, DOTA-cycratide, and linear-pep. Data are shown as mean ± SD, n = 4. (C) Binding of 68Ga-cycratide to BxPC-3 with or without blocking of cold cycratide or linear-pep. %AD/106 cells = percentage of total added dose per million cells. Data are shown as mean ± SD, n = 4. (D) Metabolic stability of 68Ga-cycratide in blood and urine of BALB/c mice (data are representative of 3 independent experiments). **P < 0.01.

Article Snippet: After endogenous peroxidase activity had been abolished using 0.3% hydrogen peroxide and antigen had been retrieved by microwave, tumor tissues were incubated with rabbit antihuman integrin α v β 6 antibody (bs-5791r; Bioss) overnight at 4°C.

Techniques: Inhibition, Binding Assay, Blocking Assay

Journal: Journal of Nuclear Medicine

Article Title: Clinical Translation of a 68 Ga-Labeled Integrin α v β 6 –Targeting Cyclic Radiotracer for PET Imaging of Pancreatic Cancer

doi: 10.2967/jnumed.119.237347

Figure Lengend Snippet: (A) PET imaging of orthotopic pancreatic cancer lesions in nude mice at 0.5 h after injection of 68Ga-cycratide without or with blocking dose of cold cycratide. Tumors are indicated by arrows. (B) Hematoxylin and eosin (H&E) staining of tumor tissues harvested from orthotopic tumor model. (C) Left, immunofluorescence staining of integrin αvβ6 from tumor tissues harvested from orthotopic tumor model. Right, negative control with secondary antibody only.

Article Snippet: After endogenous peroxidase activity had been abolished using 0.3% hydrogen peroxide and antigen had been retrieved by microwave, tumor tissues were incubated with rabbit antihuman integrin α v β 6 antibody (bs-5791r; Bioss) overnight at 4°C.

Techniques: Imaging, Injection, Blocking Assay, Staining, Immunofluorescence, Negative Control

Journal: Journal of Nuclear Medicine

Article Title: Clinical Translation of a 68 Ga-Labeled Integrin α v β 6 –Targeting Cyclic Radiotracer for PET Imaging of Pancreatic Cancer

doi: 10.2967/jnumed.119.237347

Figure Lengend Snippet: (A) PET/CT images of female patient with suspected pancreatic cancer. Images were obtained at 1 h after intravenous administration of 68Ga-cycratide or 18F-FDG. Tumors are indicated by arrows. (B) Immunohistochemical (IHC) staining for integrin αvβ6 in tumor sample from same patient as in A. (C) Contrast-enhanced CT (CECT) image and PET/CT images of male patient with pancreatic cancer 7 mo after surgery at 1 h after administration of 68Ga-cycratide or 18F-FDG, as well as CECT image of same patient 3 mo later (10 mo after surgery). Occupancy lesions in CT are indicated by arrows.

Article Snippet: After endogenous peroxidase activity had been abolished using 0.3% hydrogen peroxide and antigen had been retrieved by microwave, tumor tissues were incubated with rabbit antihuman integrin α v β 6 antibody (bs-5791r; Bioss) overnight at 4°C.

Techniques: Positron Emission Tomography-Computed Tomography, Immunohistochemical staining, Immunohistochemistry

Journal: The American Journal of Pathology

Article Title: Expression and Potential Role of Fas-Associated Phosphatase-1 in Ovarian Cancer

doi: 10.1016/s0002-9440(10)64084-9

Figure Lengend Snippet: Figure 2. Comparison of FAP-1 protein levels in ovarian cancer cell lines. Lysates from cell lines (100 g total protein) were subjected to SDS-PAGE/ immunoblot assay using rabbit polyclonal no. 1730. Jurkat and HEK 293 cells served as negative and positive controls, respectively. A nonspecific band is also observed (asterisk), serving as a loading control. Densitometrical anal- ysis was performed using a Multi Image light cabinet and the ChemiImager software version 4000 yielding values based on integrated density (#). Data were arbitrarily normalized relative to FAP-1 levels of HEK 293 cells that were included on all blots as an internal control.

Article Snippet: To quantify immunoblotting results, films were analyzed densitometrically using a Multi Image Light Cabinet and the ChemiImager software version 4000 (Alpha Innotech Corporation, San Leandro, CA).

Techniques: Comparison, SDS Page, Western Blot, Control, Software

Journal: Life Sciences

Article Title: Remdesivir: A potential game-changer or just a myth? A systematic review and meta-analysis ☆

doi: 10.1016/j.lfs.2020.118663

Figure Lengend Snippet: Qualitative analysis.

Article Snippet: Pasquini et al. [ ], retrospective observational study, Italy , N = 51 T = 25C = 26 M = 47 F = 4 Median age = 47 Inclusion criteria • Age more than 18 years • Positive RT-PCR essay • Severe respiratory failure with the need for mechanical ventilation Exclusion criteria • Mortality within the first 48 h , First dose of 200 mg IV on Day 1, plus 100 mg daily from Day 2 to Day 10 in the treatment group Concomitant therapies include hydroxychloroquine, tocilizumab and lopinavir/ritonavir , , Better survival with remdesivir using Charlson Comorbidity Index OR 3.506 (95% CI 1.768–6.954) Median follow up 52 days (46–57).

Techniques: Infection, Imaging, Polymerase Chain Reaction, Computed Tomography

Journal: European journal of nuclear medicine and molecular imaging

Article Title: Preclinical evaluation of FAP-2286 for fibroblast activation protein targeted radionuclide imaging and therapy.

doi: 10.1007/s00259-022-05842-5

Figure Lengend Snippet: Fig. 3 Correlation of FAP expression analysis by immunohistochem- istry and autoradiography using 111In-FAP-2286. Representative images of patient and mouse xenograft tumors are shown by autora- diography (left, 500 µm) and immunohistochemistry (right, 100 μm) (A). FAP levels by autoradiography demonstrate correlation to immu- nohistochemistry in patient cholangiocarcinoma and sarcoma tumor sections (Pearson correlation coefficient r = 0.79, P = 0.002) (B)

Article Snippet: In vitro assays Surface plasmon resonance assay (SPR) The binding kinetics of FAP-2286 to antibody-immobilized human FAP (Sino Biological) or mouse FAP (R&D Systems) was measured by SPR and calculated using singlecycle kinetic measurements.

Techniques: Expressing, Autoradiography, Immunohistochemistry

Journal: European journal of nuclear medicine and molecular imaging

Article Title: Preclinical evaluation of FAP-2286 for fibroblast activation protein targeted radionuclide imaging and therapy.

doi: 10.1007/s00259-022-05842-5

Figure Lengend Snippet: Fig. 4 Imaging of HEK-FAP tumor-bearing mice with 111In- FAP-2286. SPECT images of one representative mouse at 5 different timepoints are shown (A). Quantification of 111In- FAP-2286 uptake as mean ± SD %ID/g (n = 9) in tumor, liver, kidney, and blood pool surro- gate at various timepoints after injection (B)

Article Snippet: In vitro assays Surface plasmon resonance assay (SPR) The binding kinetics of FAP-2286 to antibody-immobilized human FAP (Sino Biological) or mouse FAP (R&D Systems) was measured by SPR and calculated using singlecycle kinetic measurements.

Techniques: Imaging, Single Photon Emission Computed Tomography, Injection